A Ph.D. thesis at the College of Agriculture, University of Basra discussed the increasing of tolerability of Chrysanthemum hortorum Hort. to environmental stress via a plant tissue culture technique.
The thesis presented by Ph.D. student, Lamia Hussein Musa Aish Mazine, included a study of increasing the environmental tolerability of Chrysanthemum hortorum Hort. by determining the optimal concentrations of growth regulators as well as by the chemical tensile compounds of sucrose, alcoholic sugar and the polyethylene clycode that are added to the MS medium which is prepared for the multiplication and rooting of vegetative branches, stimulating callus and proliferating it and acclimatizing plantlets.
The thesis dealt with the treating of callus with polyethylene glycol, sucrose and sorbitol at different levels to determine the best concentration for callus growth and increasing its tolerance to water tension and urging it to increase some of active compounds
The thesis aims to abundant production of this commercial plant and then the production of plants free of viral and fungal diseases and determination of the optimal combination of growth regulators.
