The College of Education for Pure Sciences in the Department of Life Sciences at the University of Basrah discussed a doctoral thesis on (a phenotypic and molecular diagnostic study of some stored grain insects in Maysan Governorate)
The thesis presented by researcher (Fatima Qasim Hamdan) included the current study surveying and isolating insects present in stored grains collected from different areas of Maysan Governorate. The thesis addressed that there are eight types of insect pests that infect stored grains, and the special phenotypic characteristics of the whole organisms were studied, which included The head, thorax, abdomen, legs and wings were also studied, which included extracting DNA and using a general primer specific to invertebrates to detect the Cytochrome oxidase subunit I gene, and sending samples after amplifying the gene to Korea for the purpose of reading the sequences of these species for the purpose of comparing these species with species distributed throughout Various types of insect pests were used in different parts of the world, and Multiplex PCR was used for early detection of six types of these insect pests based on incomplete stages.
The thesis concluded
There is a great similarity in the distribution of species found in Maysan Governorate with Basra Governorate, and these species are numerous with what was recorded in the governorates of Baghdad, Holy Karbala, Najaf Al-Ashraf, Diwaniyah, Diyala, Salah al-Din and Nineveh, and some species were not recorded during the study period despite being recorded in Other provinces are due to differences in environmental conditions and the study period in addition to other factors.
The success of Multiplex PCR technology in detecting internal infections affecting stored grains by designing primers specific to each type.
Molecular diagnostic technology is an effective and rapid means of diagnosing and confirming the diagnosis of species that are difficult to diagnose phenotypically or are closely similar to other species.
