A PhD thesis at Basraha University to be discussed (Cloning of Human interferon-gamma gene and its expression in E. coli and S. cerevisiae)
A Ph. D thesis at the department of biology at the collage of science at the University of Basraha (Cloning of Human interferon-gamma gene and its expression in E. coli and S. cerevisiae), and showed the researcher's thesis (Najim Mohsen Aziz) the importance of biopharmaceutical therapy that produced by bacteria and yeast cells compared to chemotherapy. Thesis aims to the isolation of ribonucleic acid from white blood cells and obtaining the cDNA using the reverse transcription enzyme for the purpose of obtaining the interferon-gamma gene. The interferon gene was also syntheses by the Genscript company dependent on the gene sequence in the NCBI with specific features for the purpose of comparing. The thesis aims to obtain strains of bacteria (E. coli BL21) and yeast (S. cerivisiae BY4741) that have the ability to produce interferon-gamma gene. The sonication was utilized to break down the cell wall and release the protein material for the purpose of purification. The resulting protein was purified by nickel column. The biological activity of the resulting protein was also studied by flow cytometry. The thesis concluded that the strain mutants have the ability to produce the interferon-gamma protein, which have biological activity compared to the commercial protein. The study recommended conducting several researches to prove the biological activity of the resulting protein, as well as focusing on the purity of the product for the purpose of using it as a biological treatment for some diseases.
