University of Basra master's thesis on serologic and molecular detection of hepatitis virus
A master's thesis at the University of Basra's Faculty of Science has investigated the serological and molecular detection of the hepatitis C and B virus among patients in the southern Iraqi city of Basra.
The thesis, submitted by student researcher Nour Aqeel Khairi, aimed to focus on HCV and HBV genotypes from a total of 200 patients.
The thesis included verification of the samples by enzyme-linked immunosorbent assay (ELISA). The DNA extraction and purity of the HCV and HBV genomes were verified using a Nanodrop spectrophotometer. HBV was detected by molecular detection, which was done via polymerase chain reaction (PCR) genotyping, by amplifying both the X and S genes.
The results indicated that HBV genotype C was predominant; followed by genotypes D, B, F, and I. This study also contributed to the investigation of molecular detection, genotypes, and risk factors associated with HCV infection.
Department of Media and Government Communication
