Master’s Thesis at the University of Basrah Explores the Isolation, Purification, and Characterization of Bioactive Compounds from Turmeric Using HPLC and LCMS
The College of Education for Qurna at the University of Basrah examined a master’s thesis entitled “Isolation, Purification, and Characterization of Bioactive Compounds from Turmeric Using HPLC and LCMS, and Evaluation of Their Biological Activity.”
The study, presented by graduate student Abbas Hadi Saleh, aimed to develop a safe, rapid, and cost-effective method for isolating bioactive compounds from turmeric rhizomes using HPLC technology, followed by purification to a high purity level of 99%, while obtaining sufficient quantities of the compounds after drying. The compounds were then characterized using LCMS, in addition to assessing their biological activity.
The findings demonstrated that the adopted isolation and purification method exhibited high efficiency, yielding pure and accurately identified compounds. These compounds showed strong inhibitory activity against pathogenic bacteria, both Gram-positive and Gram-negative, as well as fungi isolated from gynecological infections. Furthermore, they exhibited a positive impact in suppressing the growth of cancer cells.
The thesis recommended conducting further research to isolate additional compounds from both alcoholic and aqueous extracts of turmeric and to compare their properties. It also emphasized the need to enhance the sensitivity and accuracy of HPLC and LCMS techniques through the use of innovative phases and columns, as well as to evaluate the effectiveness of these compounds in vivo.
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