A doctoral thesis at University of Basrah examines the molecular diagnosis of Escherichia coli germ.

A doctoral thesis at University of Basrah examines the molecular diagnosis of Escherichia coli germ.

A doctoral thesis in College of Veterinary Medicine at University of Basrah examined the molecular diagnosis of Escherichia coli germ that producing colicin and isolated from stool samples and its ability to inhibit the growth of other pathogenic germs and plasmid transfer.

The thesis presented by the student Aamal Fadhil Ghanim Sekhi aims to extract the colicin protein produced by non-pathogenic intestinal bacteria and then use it as a treatment that kills pathogenic bacteria.

The thesis included isolating the aforementioned bacteria and diagnosing it using several laboratory methods, then stimulating it to produce protein then extracting protein from it, where five genes responsible for the production of the colicin protein were examined. One of the isolates was chosen as a representative strain to test these five protein genes. After using PCR, sequencing, docking simulation, cloning technology and other molecular techniques, the required protein gene was multiplied and so producing the required protein.

The thesis concluded that E.coli bacteria can be obtained abundantly from the humans and animals intestines. The protein colicin can be used as an antibacterial, as it has a noticeable effect on pathogenic Escherichia and other species.

The thesis recommended taking strict restrictions on environmental contamination with fecal matter, as it is not free of pathogenic bacteria, and non-pathogenic bacteria. It also recommended conducting extensive tests on proteins secreted by bacteria before using them as effective antibiotics.

Department of Media and Government Communication