University of Basrah discusses Gene Expression Associated with Rotavirus Infection

The Faculty of Science organized a seminar entitled "Gene expression associated with Rotavirus Infection".

The researcher Ahmed Jasim Muhammed expressed that the study included measuring the gene expression associated with Rotavirus, which is related to the stimulation of Interferons and the proteins that control its production, such as Promyelocytic Leukemia Proteins (PML) proteins. The role of PML as one of the most important Interferon-stimulating genes, which has great importance as antiviral drugs, consists of 9 exons and the result of alternative splicing. During PML Gene cloning, 7 forms of PML appeared.

The research also included mechanisms to stimulate interferon induced by the virus entering the cell through Interferon regulatory factors 3 and 7 and nuclear factor NF-kB. The virus was diagnosis genetically using PCR and the Quick Profile serological teat. The gene expression of PML, PMLII, and IFNY proteins with anti-viral capacity by RNA extraction from blood samples collected with the excrement samples for the same diabetic child, then around RNA to cDNA and entered into the Real Time PCR device to measure its gene expression (PML, PMLII, IFNY), all of which showed a significant reduction in their levels.